Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NGAL Double Nickase Plasmid (h): sc-401838-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NGAL Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NGAL Double Nickase Plasmid (h) and NGAL Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LCN2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NGAL Antibody (H-4): sc-518095
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NGAL Double Nickase Plasmid (h) | sc-401838-NIC | 20 µg | $410.00 | |||
NGAL Double Nickase Plasmid (h2) | sc-401838-NIC-2 | 20 µg | $410.00 |
LCN2 encodes neutrophil gelatinase–associated lipocalin (NGAL), a secreted lipocalin that binds siderophore-associated iron and modulates iron trafficking, antimicrobial defense, and epithelial stress responses. NGAL participates in innate immune signaling and inflammatory remodeling, intersecting with pathways controlling chemotaxis, cytokine networks, and extracellular matrix dynamics through interactions with partners such as MMP9. In human tissues, altered LCN2/NGAL expression is frequently associated with metabolic inflammation, renal and cardiovascular injury responses, and tumor–stroma communication, making it a useful readout of stress-adaptive programs. These features support mechanistic studies of iron-dependent oxidative stress, epithelial–immune crosstalk, and microenvironmental regulation.
NGAL Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LCN2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LCN2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LCN2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LCN2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.