Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NF90 Double Nickase Plasmid (h): sc-402626-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NF90 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NF90 Double Nickase Plasmid (h) and NF90 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ILF3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NF90 Antibody (A-3): sc-377406
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NF90 Double Nickase Plasmid (h) | sc-402626-NIC | 20 µg | $410.00 | |||
NF90 Double Nickase Plasmid (h2) | sc-402626-NIC-2 | 20 µg | $410.00 |
ILF3 encodes NF90, a double-stranded RNA-binding protein that functions in post-transcriptional gene regulation by modulating mRNA stability, splicing, nuclear export, and translation, often in conjunction with NF45 and other ribonucleoprotein partners. NF90 participates in innate antiviral responses and stress-adaptive programs by influencing interferon-related transcripts and RNA metabolism pathways, and it can also impact cell-cycle control through selective regulation of growth-associated mRNAs. Through its interactions with transcriptional and RNA processing machinery, NF90 helps coordinate gene expression programs linked to proliferation, differentiation, and cellular stress signaling. Dysregulation of ILF3/NF90 has been associated with altered RNA homeostasis in contexts relevant to tumor biology and inflammatory phenotypes, making it a useful node for mechanistic studies of RNA-driven regulation.
NF90 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ILF3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ILF3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ILF3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ILF3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.