neuropilin-1 Double Nickase Plasmid (m): sc-421964-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- neuropilin-1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- neuropilin-1 Double Nickase Plasmid (m) and neuropilin-1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Nrp1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: neuropilin-1 Antibody (A-12): sc-5307
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
neuropilin-1 Double Nickase Plasmid (m) | sc-421964-NIC | 20 µg | $410.00 |
Mouse Nrp1 encodes neuropilin-1, a multifunctional co-receptor that modulates signaling by class 3 semaphorins and VEGF family ligands to coordinate axon guidance, angiogenesis, and lymphatic and cardiovascular development. Neuropilin-1 integrates with VEGFR2/FLT1 and plexin complexes to shape downstream MAPK/ERK, PI3K–AKT, and Rho GTPase-dependent cytoskeletal dynamics, influencing migration, adhesion, and vascular permeability. In immune and stromal compartments, NRP1 contributes to cell–cell communication and tissue remodeling through effects on dendritic cell and regulatory T cell biology and on extracellular matrix interactions. Dysregulated NRP1 signaling is associated with pathological neovascularization, inflammation-driven tissue remodeling, and tumor microenvironment processes, making it a relevant node for mechanistic studies of vascular and guidance pathways.
neuropilin-1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Nrp1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Nrp1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Nrp1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Nrp1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.