Neurofibromin 2/NF2/Merlin Double Nickase Plasmid (h): sc-400504-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Neurofibromin 2/NF2/Merlin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Neurofibromin 2/NF2/Merlin Double Nickase Plasmid (h) and Neurofibromin 2/NF2/Merlin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NF2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Neurofibromin 2/NF2/Merlin Antibody (B-12): sc-55575
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Neurofibromin 2/NF2/Merlin Double Nickase Plasmid (h) | sc-400504-NIC | 20 µg | $410.00 | |||
Neurofibromin 2/NF2/Merlin Double Nickase Plasmid (h2) | sc-400504-NIC-2 | 20 µg | $410.00 |
NF2 encodes neurofibromin 2 (Merlin), a membrane–cytoskeleton adaptor that integrates signals from cell–cell junctions and the actin cortex to constrain proliferation and maintain epithelial architecture. Merlin functions as a tumor suppressor by regulating contact inhibition and modulating pathways including Hippo-YAP/TAZ, receptor tyrosine kinase signaling, and cytoskeletal dynamics that influence migration and mechanical signaling. Loss or inactivation of NF2 disrupts these growth-control networks, promoting aberrant cell cycle entry and altered adhesion-dependent signaling. NF2 dysfunction is strongly linked to neurofibromatosis type 2 and Merlin-deficient tumors such as schwannomas and meningiomas, making it a key node for studying growth regulation and mechanotransduction in human cells.
Neurofibromin 2/NF2/Merlin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NF2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NF2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NF2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NF2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.