NCAM/CD56 Double Nickase Plasmid (h): sc-400302-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NCAM/CD56 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NCAM/CD56 Double Nickase Plasmid (h) and NCAM/CD56 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NCAM1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NCAM/CD56 Antibody (123C3): sc-7326
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NCAM/CD56 Double Nickase Plasmid (h) | sc-400302-NIC | 20 µg | $410.00 | |||
NCAM/CD56 Double Nickase Plasmid (h2) | sc-400302-NIC-2 | 20 µg | $410.00 |
NCAM1 encodes the neural cell adhesion molecule NCAM/CD56, an immunoglobulin superfamily glycoprotein that mediates Ca²⁺-independent cell–cell and cell–matrix interactions. Through homophilic and heterophilic binding, NCAM/CD56 regulates neurite outgrowth, axon guidance, synaptic plasticity, and migration, and it can signal via Fyn/FAK, MAPK/ERK, and cytoskeletal remodeling pathways. Alternative splicing and polysialylation modulate adhesive strength and dynamic cell motility during development and tissue repair. Dysregulated NCAM1 expression or modification is linked to altered neurodevelopmental processes and is also used as a functional marker in NK cell biology and studies of tumor cell invasion and immune interactions.
NCAM/CD56 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NCAM1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NCAM1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NCAM1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NCAM1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.