Nanog Antibody (F-8): sc-514536

Nanog Antibody (F-8) is a mouse monoclonal IgM kappa light chain antibody engineered to detect Nanog protein of mouse origin across various applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Nanog antibody (F-8) is raised against a specific epitope mapping between amino acids 233-256 near the C-terminus of mouse Nanog protein, ensuring accurate recognition and binding to the targeted region. Nanog, also called Nanog homeobox, is a critical homeodomain-containing transcription factor that plays an essential role in maintaining pluripotency and self-renewal of embryonic stem cells, thereby preventing differentiation into specialized cell types. Located predominantly in the nucleus, Nanog regulates the expression of key genes that sustain the undifferentiated state of stem cells, making nuclear localization crucial for function in orchestrating gene expression programs essential for pluripotency. Nuclear positioning of Nanog enables interaction with other transcription factors and regulatory proteins, forming complexes that control transcriptional networks necessary for maintaining stem cell identity and facilitating cellular reprogramming. By targeting these specific functional regions, Nanog (F-8) monoclonal antibody enables researchers to accurately study localization, interaction, and regulation of Nanog within cells, providing valuable insights into mechanisms that govern stem cell maintenance and differentiation. Anti-Nanog antibody (F-8) serves as an indispensable tool for investigations in stem cell biology, developmental biology, and regenerative medicine, facilitating advancements in understanding how pluripotent states are preserved and manipulated for therapeutic purposes.

The preferred Nanog antibody is Nanog Antibody (5A10): sc-134218.