Munc13-2 CRISPR/Cas9 KO Plasmid (m): sc-423611
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Munc13-2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Munc13-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Munc13-2 CRISPR/Cas9 KO Plasmid (m) | sc-423611 | 20 µg | $397.00 |
Overview
Unc13b encodes Munc13-2, a presynaptic active zone priming factor required for maturation of synaptic vesicles into a readily releasable state and efficient Ca²⁺-evoked neurotransmitter release. Munc13-2 cooperates with SNARE regulators and the RIM/Rab3 network to coordinate docking, priming, and short-term plasticity, thereby shaping synaptic strength and circuit activity. Through its C1 and C2 domains, Munc13-2 integrates diacylglycerol- and Ca²⁺-dependent signaling to tune release probability and vesicle pool dynamics. Dysregulation of vesicle priming and synaptic transmission is broadly relevant to neurodevelopmental and neuropsychiatric phenotypes, motivating mechanistic studies of Unc13b in neuronal signaling and network dysfunction.
Munc13-2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Unc13b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Unc13b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Unc13b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Munc13-2 protein expression.
This CRISPR knockout system enables efficient generation of Unc13b-deficient cell models for investigation of Munc13-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Unc13b exon(s) critical for Munc13-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Unc13b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Munc13-2 CRISPR/Cas9 KO Plasmid (m) and Munc13-2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Unc13b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Munc13-2 HDR Plasmid (m) and Munc13-2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Unc13b homology arms to support homology-directed repair at defined Unc13b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.