MRP1 Antibody (QCRL-2): sc-18836

MRP1 Antibody (QCRL-2) is a mouse monoclonal IgG2b antibody that detects MRP1 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). MRP1, or multidrug-resistance protein 1, plays a crucial role in the cellular efflux of a wide range of substrates, including drugs and toxins, thereby contributing to the phenomenon of multidrug resistance in cancer cells. MRP1 is primarily located in the plasma membrane of various cell types, including those in the brain, lungs, and peripheral blood, which is significant because MRP1 expression in these tissues can influence drug pharmacokinetics and therapeutic efficacy. MRP1 presence in the blood-brain barrier can limit the effectiveness of chemotherapeutic agents in treating brain tumors, making MRP1 a critical target for research aimed at overcoming drug resistance. MRP1′s structure includes an Mdr-like core and a unique N-terminal membrane-bound region, which are essential for MRP1 function as a transporter. Understanding MRP1 dynamics and interactions with other proteins can provide insights into the mechanisms of drug resistance and potential strategies for enhancing drug delivery in resistant cancers.

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MRP1 Antibody (QCRL-2) References:

1. ATP- and glutathione-dependent transport of chemotherapeutic drugs by the multidrug resistance protein MRP1.  |  Renes, J., et al. 1999. Br J Pharmacol. 126: 681-8. PMID: 10188979
2. Expression of the multidrug resistance protein (MRP1) in breast cancer.  |  Filipits, M., et al. 1999. Anticancer Res. 19: 5043-9. PMID: 10697508
3. Verapamil stimulates glutathione transport by the 190-kDa multidrug resistance protein 1 (MRP1).  |  Loe, DW., et al. 2000. J Pharmacol Exp Ther. 293: 530-8. PMID: 10773025
4. Functional reconstitution of substrate transport by purified multidrug resistance protein MRP1 (ABCC1) in phospholipid vesicles.  |  Mao, Q., et al. 2000. J Biol Chem. 275: 34166-72. PMID: 10942765
5. Influences of glutathione on anionic substrate efflux in tumour cells expressing the multidrug resistance-associated protein, MRP1.  |  Bagrij, T., et al. 2001. Biochem Pharmacol. 62: 199-206. PMID: 11389878
6. Flow cytometric monitoring of multidrug drug resistance protein 1 (MRP1/ABCC1) -mediated transport of 2',7'-bis-(3-carboxypropyl)-5-(and-6)- carboxyfluorescein (BCPCF) into human erythrocyte membrane inside-out vesicles.  |  Bobrowska-Hägerstrand, M., et al. 2007. Mol Membr Biol. 24: 485-95. PMID: 17710652
7. The expression and significance of MRP1, LRP, TOPOIIβ, and BCL2 in tongue squamous cell carcinoma.  |  Zhang, B., et al. 2012. J Oral Pathol Med. 41: 141-8. PMID: 21793937
8. Differential functional rescue of Lys(513) and Lys(516) processing mutants of MRP1 (ABCC1) by chemical chaperones reveals different domain-domain interactions of the transporter.  |  Iram, SH. and Cole, SP. 2014. Biochim Biophys Acta. 1838: 756-65. PMID: 24231430
9. Sequences in Linker-1 domain of the multidrug resistance associated protein (MRP1 or ABCC1) bind to tubulin and their binding is modulated by phosphorylation.  |  Ambadipudi, R. and Georges, E. 2017. Biochem Biophys Res Commun. 482: 1001-1006. PMID: 27908733
10. Recent Advances in Photothermal Therapies Against Cancer and the Role of Membrane Transporter Modulators on the Efficacy of This Approach.  |  Al-Ali, AAA., et al. 2023. Technol Cancer Res Treat. 22: 15330338231168016. PMID: 37138532

The preferred MRP1 antibody is MRP1 Antibody (QCRL-1): sc-18835.