MIF Antibody (D-2): sc-271631

MIF Antibody (D-2) is a mouse monoclonal IgM antibody that detects macrophage migration inhibitory factor (MIF) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MIF Antibody (D-2) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. MIF, also known as glycosylation-inhibiting factor, is a secreted, homotrimeric pro-inflammatory cytokine that plays a crucial role in modulating macrophage and T cell functions, making MIF an essential regulator of host immune response to infection. MIF is primarily produced by the pituitary gland and is expressed in various cell types, including monocytes, macrophages, and differentiating immunological cells in the eye lens and brain, as well as fibroblasts. Notably, MIF is found at sites of inflammation, indicating MIF′s significant role in regulating macrophage activity during host defense. Elevated levels of MIF have been associated with severe sepsis and septic shock, where MIF enhances the production of other inflammatory cytokines such as tumor necrosis factor α, interleukin-1, and interferon-γ, thereby promoting a systemic inflammatory response. Furthermore, MIF may contribute to tissue damage by inducing proteinases, highlighting MIF′s dual role in both immune regulation and potential pathophysiological processes.

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MIF Antibody (D-2) References:

1. High expression of macrophage migration inhibitory factor in the synovial tissues of rheumatoid joints.  |  Onodera, S., et al. 1999. Cytokine. 11: 163-7. PMID: 10089139
2. Pro-1 of macrophage migration inhibitory factor functions as a catalytic base in the phenylpyruvate tautomerase activity.  |  Lubetsky, JB., et al. 1999. Biochemistry. 38: 7346-54. PMID: 10353846
3. Protection from septic shock by neutralization of macrophage migration inhibitory factor.  |  Calandra, T., et al. 2000. Nat Med. 6: 164-70. PMID: 10655104
4. Macrophage migration inhibitory factor (MIF) as a paracrine mediator in the interaction of testicular somatic cells.  |  Meinhardt, A., et al. 2000. Andrologia. 32: 46-8. PMID: 10702866
5. The proinflammatory mediator macrophage migration inhibitory factor induces glucose catabolism in muscle.  |  Benigni, F., et al. 2000. J Clin Invest. 106: 1291-300. PMID: 11086030
6. Macrophage migration inhibitory factor (MIF) modulates innate immune responses induced by endotoxin and Gram-negative bacteria.  |  Roger, T., et al. 2001. J Endotoxin Res. 7: 456-60. PMID: 11753217
7. Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor.  |  Weiser, WY., et al. 1989. Proc Natl Acad Sci U S A. 86: 7522-6. PMID: 2552447
8. MIF Promotes Classical Activation and Conversion of Inflammatory Ly6C(high) Monocytes into TipDCs during Murine Toxoplasmosis.  |  Ruiz-Rosado, Jde D., et al. 2016. Mediators Inflamm. 2016: 9101762. PMID: 27057101
9. Macrophage migration inhibitory factor (MIF) enhances hypochlorous acid production in phagocytic neutrophils.  |  Schindler, L., et al. 2021. Redox Biol. 41: 101946. PMID: 33823474
10. The N-terminus of MIF regulates the dynamic profile of residues involved in CD74 activation.  |  Parkins, A., et al. 2021. Biophys J. 120: 3893-3900. PMID: 34437846
11. Purification, bioactivity, and secondary structure analysis of mouse and human macrophage migration inhibitory factor (MIF).  |  Bernhagen, J., et al. 1994. Biochemistry. 33: 14144-55. PMID: 7947826
12. Cloning the human gene for macrophage migration inhibitory factor (MIF).  |  Paralkar, V. and Wistow, G. 1994. Genomics. 19: 48-51. PMID: 8188240

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MIF Antibody (D-2) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-MIF Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MIF Antibody (D-2) Specifications

Alternate Product Name: MIF

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 7-39

Epitope Target: MIF

Epitope Species: human

Product Reactivity/Detection: MIF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for MIF Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin