Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MCM2 Double Nickase Plasmid (h): sc-400959-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MCM2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MCM2 Double Nickase Plasmid (h) and MCM2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MCM2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MCM2 Antibody (E-8): sc-373702
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MCM2 Double Nickase Plasmid (h) | sc-400959-NIC | 20 µg | $410.00 | |||
MCM2 Double Nickase Plasmid (h2) | sc-400959-NIC-2 | 20 µg | $410.00 |
MCM2 encodes a core subunit of the minichromosome maintenance (MCM2–7) helicase complex that licenses DNA replication origins and drives replication fork progression during S phase. Through coordinated action with CDC45 and the GINS complex, MCM2 supports replisome assembly, replication stress responses, and coupling of DNA synthesis to checkpoint signaling. Disruption or dysregulated expression of MCM2 can perturb genome stability, elevate replication stress, and alter cell-cycle control programs frequently studied in proliferative disorders. As a marker of cycling cells, MCM2 is widely used to interrogate replication dynamics, chromatin-bound licensing states, and mechanisms of DNA damage tolerance.
MCM2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MCM2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MCM2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MCM2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MCM2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.