Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MBNL2 Double Nickase Plasmid (h): sc-401921-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MBNL2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MBNL2 Double Nickase Plasmid (h) and MBNL2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MBNL2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MBNL2 Antibody (3B4): sc-136167
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MBNL2 Double Nickase Plasmid (h) | sc-401921-NIC | 20 µg | $410.00 | |||
MBNL2 Double Nickase Plasmid (h2) | sc-401921-NIC-2 | 20 µg | $410.00 |
MBNL2 (muscleblind-like splicing regulator 2) is an RNA-binding protein that recognizes YGCY motifs to control alternative splicing, mRNA localization, and stability across development and tissue differentiation. In the nucleus it coordinates splice-site selection and contributes to RNA processing networks that shape cytoskeletal organization, cell-cycle progression, and stress-adaptive transcript programs. MBNL2 is widely studied in the context of RNA repeat–associated toxicity and spliceopathy mechanisms, where perturbation of MBNL family activity drives widespread mis-splicing. Dysregulated MBNL2-dependent splicing has been linked to neuromuscular and neurodegenerative disease models, making it a key target for dissecting RNA-processing pathways.
MBNL2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MBNL2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MBNL2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MBNL2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MBNL2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.