LIF (mBA): sc-4989. LIFを含まない0. 3％寒天培地で1週間培養した未分化マウスM1細胞 (A) と, 0. 05ng/mlのマウスLIFを含む0. 3％寒天培地で1週間培養した分化マウスM1細胞 (B).
LIF (mBA): sc-4989
- マウス由来のマチュア白血病阻止因子（LIF）、凍結乾燥された
- available as 106 units (10 µg) (sc-4989) or 5x106 units (50 µg) (sc-4989A)
- potent inhibitor of spontaneous embryonic mouse stem cell differentiation and useful for in vitro maintenance of the pluripotential phenotype of ES cells
- 1000 U per 1 ml of tissue culture media is required to maintain mouse ES cells with a stem cell phenotype; 106 units is sufficient for 1 L of tissue culture media, 107 units is sufficient for 10 L of tissue culture media
- produced in E. coli as a GST-tagged fusion protein; GST tag is cleaved and LIF is purified by HPLC chromatography
- >95% pure by SDS-PAGE and 0.22 micron sterile filtered prior to lyophilization; tested negative for mycoplasmas
- Mature, biologically active, recombinant LIF is indistinguishable from native LIF in its biological activities in vitro.
- Leukemia Inhibitory Factor (LIF) is an important cell culture supplement. LIF suppresses spontaneous differentiation thereby promoting long-term maintenance of embryonic stem cells. Additional LIF functions include stem cell pluripotency control, cholinergic neuron differentiation, metabolism of bone and fat, promotion of megakaryocyte production and mitogenesis of certain factor dependent cell lines in vivo.
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| 製品名 | カタログ # | 単位 | 価格 | 数量 | お気に入り | |
LIF (mBA) | sc-4989 | 106 units (10 µg) | $272.00 | |||
LIF (mBA) | sc-4989A | 5x106 units (50 µg) | $470.00 |