Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LHX1 Double Nickase Plasmid (h): sc-403100-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LHX1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LHX1 Double Nickase Plasmid (h) and LHX1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LHX1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LHX1 Antibody (C-6): sc-515631
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LHX1 Double Nickase Plasmid (h) | sc-403100-NIC | 20 µg | $410.00 | |||
LHX1 Double Nickase Plasmid (h2) | sc-403100-NIC-2 | 20 µg | $410.00 |
LHX1 (LIM homeobox 1) encodes a LIM domain–containing homeodomain transcription factor that coordinates tissue patterning and cell fate decisions during embryonic development. In human cells, LHX1 regulates lineage-specific transcriptional programs by binding DNA and partnering with cofactors that modulate chromatin and transcriptional networks. It is implicated in developmental processes including axial patterning and urogenital system specification, linking its activity to pathways that govern differentiation and morphogenesis. Dysregulated LHX1 expression has been associated with congenital anomalies and has been reported in subsets of cancers, making it relevant for studying transcriptional control mechanisms in disease contexts.
LHX1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LHX1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LHX1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LHX1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LHX1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.