Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LC3B Double Nickase Plasmid (m): sc-426563-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LC3B Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LC3B Double Nickase Plasmid (m) and LC3B Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Map1lc3b. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LC3B Antibody (G-9): sc-376404
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LC3B Double Nickase Plasmid (m) | sc-426563-NIC | 20 µg | $410.00 | |||
LC3B Double Nickase Plasmid (m2) | sc-426563-NIC-2 | 20 µg | $410.00 |
Mouse Map1lc3b encodes microtubule-associated protein 1 light chain 3 beta (LC3B), a core component of the autophagy machinery that undergoes lipidation to form LC3-II and associate with autophagosomal membranes. LC3B supports phagophore expansion, autophagosome maturation, and selective cargo turnover through interactions with LIR-containing receptors such as p62/SQSTM1, linking ubiquitinated substrates to autophagic sequestration. As a widely used marker of autophagic flux, LC3B is central to lysosome-dependent proteostasis pathways that intersect with mitochondrial quality control, innate immune signaling, and cellular stress responses. Dysregulation of LC3B-associated autophagy has been implicated in mechanisms relevant to neurodegeneration, inflammatory states, infection biology, and cancer cell adaptation, making Map1lc3b a common target for pathway interrogation.
LC3B Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Map1lc3b locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Map1lc3b. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Map1lc3b function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Map1lc3b-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.