LBP Double Nickase Plasmid (h): sc-402651-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LBP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- LBP Double Nickase Plasmid (h) and LBP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LBP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LBP Antibody (4E8): sc-293253
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LBP Double Nickase Plasmid (h) | sc-402651-NIC | 20 µg | $410.00 | |||
LBP Double Nickase Plasmid (h2) | sc-402651-NIC-2 | 20 µg | $410.00 |
Lipopolysaccharide binding protein (LBP) is a soluble acute-phase glycoprotein that binds bacterial lipopolysaccharide and facilitates its transfer to CD14 and the TLR4/MD-2 receptor complex, amplifying innate immune recognition of Gram-negative bacteria. Through this LPS presentation function, LBP contributes to activation of NF-κB and downstream inflammatory cytokine programs that shape leukocyte recruitment and systemic inflammatory responses. LBP expression is regulated by inflammatory cues and is often used as a marker of endotoxin exposure, linking it to studies of host–microbe interactions and immune homeostasis. Dysregulated LBP activity or abundance has been associated with inflammatory phenotypes and infection-related pathophysiology, making it relevant for mechanistic work in immunology and metabolic inflammation models.
LBP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LBP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LBP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LBP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LBP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.