Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IP-10 CRISPR Activation Plasmid (m): sc-421031-ACT
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IP-10 CRISPR Activation Plasmid (m) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- IP-10 CRISPR Activation Plasmid (m) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by IP-10 CRISPR Activation Plasmid (m) and IP-10 CRISPR Activation Plasmid (m2) target distinct regulatory regions upstream of the Cxcl10 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IP-10 Antibody (E-2): sc-374092
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IP-10 CRISPR Activation Plasmid (m) | sc-421031-ACT | 20 µg | $397.00 | |||
IP-10 CRISPR Activation Plasmid (m2) | sc-421031-ACT-2 | 20 µg | $397.00 |
Mouse Cxcl10 encodes interferon gamma–induced protein 10 (IP-10/CXCL10), a CXC chemokine that signals primarily through CXCR3 to regulate chemotaxis and positioning of activated T cells, NK cells, and other leukocyte subsets. Its expression is strongly induced downstream of IFN-γ and pattern-recognition receptor pathways, integrating JAK/STAT and NF-κB–linked inflammatory programs that shape tissue immune surveillance. IP-10 helps coordinate leukocyte trafficking, endothelial activation, and amplification of cytokine networks during infection and sterile inflammation. Dysregulated CXCL10 signaling is frequently studied in models of autoimmunity, neuroinflammation, and tumor-immune interactions where altered immune cell recruitment contributes to pathology.
IP-10 CRISPR Activation Plasmid (m) provides a targeted, non-destructive approach to upregulating endogenous Cxcl10 expression without altering the underlying DNA sequence.
IP-10 CRISPR Activation Plasmid (m) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the Cxcl10 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the Cxcl10 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous IP-10 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native Cxcl10 locus and enabling the study of IP-10-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of IP-10 pathway restoration in tumor cells with silenced or reduced Cxcl10 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.