Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
involucrin Antibody (SY5) Alexa Fluor® 647: sc-21748 AF647. Direct fluorescent western blot analysis of involucrin expression in human cervix tissue extract. Blocked with UltraCruz® Blocking Reagent: sc-516214. Cruz Marker™ Molecular Weight Standards detected with Cruz Marker™ MW Tag-Alexa Fluor® 488: sc-516790.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
involucrin Double Nickase Plasmid (h): sc-416724-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- involucrin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- involucrin Double Nickase Plasmid (h) and involucrin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IVL. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: involucrin Antibody (SY5): sc-21748
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
involucrin Double Nickase Plasmid (h) | sc-416724-NIC | 20 µg | $410.00 | |||
involucrin Double Nickase Plasmid (h2) | sc-416724-NIC-2 | 20 µg | $410.00 |