Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IK1 Double Nickase Plasmid (h): sc-402139-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IK1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IK1 Double Nickase Plasmid (h) and IK1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KCNN4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IK1 Antibody (D-5): sc-365265
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IK1 Double Nickase Plasmid (h) | sc-402139-NIC | 20 µg | $410.00 | |||
IK1 Double Nickase Plasmid (h2) | sc-402139-NIC-2 | 20 µg | $410.00 |
Human KCNN4 encodes IK1 (KCa3.1), an intermediate-conductance Ca2+-activated K+ channel that couples cytosolic Ca2+ signals to membrane hyperpolarization and K+ efflux. By shaping membrane potential and driving Ca2+ entry, IK1 influences calcium-dependent signaling, volume regulation, and downstream transcriptional programs that control proliferation, migration, and immune cell activation. IK1 activity is commonly studied in pathways involving Ca2+/calmodulin regulation, store-operated Ca2+ entry, and cytoskeletal remodeling. Dysregulated KCNN4/IK1 function has been associated with inflammatory and immune-mediated processes, vascular remodeling, and altered growth behaviors in diverse cell types, supporting its relevance to mechanistic disease modeling.
IK1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KCNN4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KCNN4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KCNN4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KCNN4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.