Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IFRD1 Double Nickase Plasmid (h): sc-405862-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IFRD1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IFRD1 Double Nickase Plasmid (h) and IFRD1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting IFRD1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IFRD1 Antibody (D-7): sc-515012
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IFRD1 Double Nickase Plasmid (h) | sc-405862-NIC | 20 µg | $410.00 | |||
IFRD1 Double Nickase Plasmid (h2) | sc-405862-NIC-2 | 20 µg | $410.00 |
Interferon-related developmental regulator 1 (IFRD1) is a transcriptional co-regulator that modulates gene expression programs linked to cell differentiation, stress responses, and inflammatory signaling. IFRD1 can influence chromatin- and transcription factor–dependent control of downstream targets, contributing to regulation of myeloid cell function and stimulus-induced transcriptional outputs. In human cells, IFRD1 has been studied in contexts involving immune regulation and tissue homeostasis, where altered expression may impact pathways governing cytokine-responsive gene networks and cellular maturation. These properties make IFRD1 a useful node for mechanistic interrogation of transcriptional control during inflammation-associated processes and differentiation states.
IFRD1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the IFRD1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within IFRD1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt IFRD1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of IFRD1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.