Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Hrs Double Nickase Plasmid (m): sc-420851-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hrs Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Hrs Double Nickase Plasmid (m) and Hrs Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Hgs. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Hrs Antibody (C-7): sc-271455
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hrs Double Nickase Plasmid (m) | sc-420851-NIC | 20 µg | $410.00 | |||
Hrs Double Nickase Plasmid (m2) | sc-420851-NIC-2 | 20 µg | $410.00 |
Mouse Hgs encodes Hrs, a core component of the ESCRT-0 complex that recognizes ubiquitinated cargo and initiates endosomal sorting toward multivesicular bodies and lysosomal degradation. Hrs coordinates receptor tyrosine kinase downregulation and signal termination, linking endocytosis to pathways such as EGFR/MAPK and PI3K–AKT signaling, and contributes to endosome maturation and membrane trafficking. Through its roles in cargo clustering and ESCRT recruitment, Hrs influences autophagy-endolysosomal crosstalk and proteostasis. Dysregulated Hrs-dependent trafficking has been associated with altered receptor signaling and neurodegenerative and oncogenic processes, making Hgs a useful node for mechanistic studies of endosomal signaling and protein turnover.
Hrs Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Hgs locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Hgs. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Hgs function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Hgs-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.