Hrs Antibody (D-3): sc-271925

Hrs Antibody (D-3) is a mouse monoclonal IgM antibody that detects hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Hrs antibody (D-3) recognizes Hrs, a crucial zinc-finger protein that plays a significant role in intracellular trafficking and signal transduction, particularly associated with early endosomes. Hrs′s unique structure includes a phosphatidylinositol 3-phosphate-binding domain, which is vital for endosomal targeting, allowing Hrs to co-localize with Clathrin through a Clathrin box motif located at its carboxy-terminus. This interaction is essential for proper functioning of endosomal sorting and trafficking processes, which are critical for maintaining cellular homeostasis and responding to extracellular signals. Additionally, Hrs is involved in ventral folding morphogenesis and shares structural similarities with yeast protein Vps27p, known for its role in vacuolar protein sorting. The human Hrs gene, located on chromosome 17q25, encodes a 777 amino acid protein, and in Schwann cells, Hrs co-localizes with tumor suppressor protein schwannomin at endosomes, indicating a potential collaborative role in Hrs-mediated cell signaling pathways.

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Hrs Antibody (D-3) References:

1. Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) mediates post-endocytic trafficking of protease-activated receptor 2 and calcitonin receptor-like receptor.  |  Hasdemir, B., et al. 2007. J Biol Chem. 282: 29646-57. PMID: 17675298
2. Loss of hrs in the central nervous system causes accumulation of ubiquitinated proteins and neurodegeneration.  |  Tamai, K., et al. 2008. Am J Pathol. 173: 1806-17. PMID: 19008375
3. The Hrs/Stam complex acts as a positive and negative regulator of RTK signaling during Drosophila development.  |  Chanut-Delalande, H., et al. 2010. PLoS One. 5: e10245. PMID: 20422006
4. Hrs controls sorting of the epithelial Na+ channel between endosomal degradation and recycling pathways.  |  Zhou, R., et al. 2010. J Biol Chem. 285: 30523-30. PMID: 20675381
5. Hrs recognizes a hydrophobic amino acid cluster in cytokine receptors during ubiquitin-independent endosomal sorting.  |  Amano, Y., et al. 2011. J Biol Chem. 286: 15458-72. PMID: 21362618
6. Hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs) is involved in BMP signaling through phosphorylation of SMADS and TAK1 in early mouse embryo.  |  Miura, S. and Mishina, Y. 2011. Dev Dyn. 240: 2474-81. PMID: 21953618
7. Regulation of hepatitis C virus secretion by the Hrs-dependent exosomal pathway.  |  Tamai, K., et al. 2012. Virology. 422: 377-85. PMID: 22138215
8. An essential role of Hrs/Vps27 in endosomal cholesterol trafficking.  |  Du, X., et al. 2012. Cell Rep. 1: 29-35. PMID: 22832105
9. ESCRT-0 protein hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is targeted to endosomes independently of signal-transducing adaptor molecule (STAM) and the complex formation with STAM promotes its endosomal dissociation.  |  Kojima, K., et al. 2014. J Biol Chem. 289: 33296-310. PMID: 25296754
10. Hrs and STAM function synergistically to bind ubiquitin-modified cargoes in vitro.  |  Takahashi, H., et al. 2015. Biophys J. 108: 76-84. PMID: 25564854

The preferred Hrs antibody is Hrs Antibody (C-7): sc-271455.