hnRNP F/H Antibody (G-8): sc-390085

hnRNP F/H Antibody (G-8) is a mouse monoclonal IgG1 antibody that detects hnRNP F/H in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). hnRNP F/H plays a crucial role in pre-mRNA processing and transport, acting as a key player in spliceosome complex. hnRNP F/H is primarily located in the nucleus, where hnRNP F/H binds to heterogeneous nuclear RNA (hnRNA) and influences pre-mRNA splicing by interacting with various splicing factors. Nuclear localization of hnRNP F/H enables precise regulation of gene expression at transcriptional level, ensuring accurate mRNA processing before protein translation. hnRNP F/H shuttles between nucleus and cytoplasm, which is essential for mRNA transport and stability. Interaction of hnRNP F/H with specific RNA sequences, particularly poly(rG) regions, highlights importance in RNA metabolism and potential implications in cellular processes, including stress responses and signaling pathways. With diverse roles in RNA processing and transport, hnRNP F/H maintains cellular homeostasis and regulates gene expression, making anti-hnRNP F/H antibody (G-8) an invaluable research tool for studying these critical biological processes.

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hnRNP F/H Antibody (G-8) References:

1. hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.  |  Expert-Bezançon, A., et al. 2004. J Biol Chem. 279: 38249-59. PMID: 15208309
2. Heterogeneous nuclear ribonucleoprotein F/H proteins modulate the alternative splicing of the apoptotic mediator Bcl-x.  |  Garneau, D., et al. 2005. J Biol Chem. 280: 22641-50. PMID: 15837790
3. Antagonistic effects of the SRp30c protein and cryptic 5' splice sites on the alternative splicing of the apoptotic regulator Bcl-x.  |  Cloutier, P., et al. 2008. J Biol Chem. 283: 21315-24. PMID: 18534987
4. Involvement of heterogeneous ribonucleoprotein F in the regulation of cell proliferation via the mammalian target of rapamycin/S6 kinase 2 pathway.  |  Goh, ET., et al. 2010. J Biol Chem. 285: 17065-76. PMID: 20308064
5. VERSE: a varying effect regression for splicing elements discovery.  |  Zhang, J., et al. 2012. J Comput Biol. 19: 855-65. PMID: 22651811
6. A targeted oligonucleotide enhancer of SMN2 exon 7 splicing forms competing quadruplex and protein complexes in functional conditions.  |  Smith, LD., et al. 2014. Cell Rep. 9: 193-205. PMID: 25263560
7. Inner nuclear envelope protein SUN1 plays a prominent role in mammalian mRNA export.  |  Li, P. and Noegel, AA. 2015. Nucleic Acids Res. 43: 9874-88. PMID: 26476453
8. SRSF10 Connects DNA Damage to the Alternative Splicing of Transcripts Encoding Apoptosis, Cell-Cycle Control, and DNA Repair Factors.  |  Shkreta, L., et al. 2016. Cell Rep. 17: 1990-2003. PMID: 27851963
9. Functional characterization of naturally occurring genetic variations of the human guanine-rich RNA sequence binding factor 1 (GRSF1).  |  Sofi, S., et al. 2018. Biochim Biophys Acta Gen Subj. 1862: 866-876. PMID: 29366917
10. Differential Conformational Dynamics Encoded by the Linker between Quasi RNA Recognition Motifs of Heterogeneous Nuclear Ribonucleoprotein H.  |  Penumutchu, SR., et al. 2018. J Am Chem Soc. 140: 11661-11673. PMID: 30122033

The preferred hnRNP F/H antibody is hnRNP F/H Antibody (1G11): sc-32310.