HNF-4α Double Nickase Plasmid (h): sc-400159-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HNF-4α Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HNF-4α Double Nickase Plasmid (h) and HNF-4α Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HNF4A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HNF-4α Antibody (Q-15): sc-101059
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HNF-4α Double Nickase Plasmid (h) | sc-400159-NIC | 20 µg | $410.00 | |||
HNF-4α Double Nickase Plasmid (h2) | sc-400159-NIC-2 | 20 µg | $410.00 |
HNF4A encodes hepatocyte nuclear factor 4 alpha (HNF-4α), a nuclear receptor–type transcription factor that controls tissue-specific gene expression programs in liver, intestine, kidney, and pancreatic islets. HNF-4α binds DNA as a homodimer to regulate networks governing glucose and lipid metabolism, bile acid homeostasis, xenobiotic and drug metabolism, and epithelial differentiation, integrating with other hepatic and endocrine regulators to maintain metabolic homeostasis. Perturbation of HNF4A-dependent transcriptional circuitry is linked to monogenic diabetes (MODY1) and broader cardiometabolic traits, and altered HNF-4α activity has been studied in contexts of hepatic steatosis, inflammatory signaling, and epithelial tumor biology. These features make HNF4A a key node for dissecting transcriptional control of metabolic and differentiation pathways in human cells.
HNF-4α Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HNF4A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HNF4A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HNF4A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HNF4A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.