Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HIP1 Double Nickase Plasmid (h): sc-402515-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HIP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HIP1 Double Nickase Plasmid (h) and HIP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HIP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HIP1 Antibody (4B10): sc-47754
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIP1 Double Nickase Plasmid (h) | sc-402515-NIC | 20 µg | $410.00 | |||
HIP1 Double Nickase Plasmid (h2) | sc-402515-NIC-2 | 20 µg | $410.00 |
Human HIP1 (huntingtin interacting protein 1) encodes an endocytic adaptor that links clathrin-coated pits to the actin cytoskeleton and coordinates trafficking of receptor tyrosine kinases and other membrane proteins. Through its ANTH domain and interactions with clathrin/AP-2, HIP1 helps regulate internalization, recycling, and signaling output from growth factor receptors, influencing pathways that control proliferation, survival, and cytoskeletal dynamics. Altered HIP1 expression or rearrangements have been reported in multiple cancers and are studied for their impact on receptor turnover and oncogenic signaling. HIP1 is also investigated in the context of proteostasis and neurobiology due to its functional connection to huntingtin-associated processes.
HIP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HIP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HIP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HIP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HIP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.