Hepatic Lipase Double Nickase Plasmid (h): sc-403928-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hepatic Lipase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Hepatic Lipase Double Nickase Plasmid (h) and Hepatic Lipase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LIPC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Hepatic Lipase Antibody (XHL3-6): sc-21740
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hepatic Lipase Double Nickase Plasmid (h) | sc-403928-NIC | 20 µg | $410.00 | |||
Hepatic Lipase Double Nickase Plasmid (h2) | sc-403928-NIC-2 | 20 µg | $410.00 |
Human LIPC encodes hepatic lipase, an endothelial-bound triglyceride lipase that hydrolyzes triglycerides and phospholipids in circulating lipoproteins, influencing HDL remodeling and the conversion of IDL to LDL. By regulating lipoprotein composition and clearance, hepatic lipase integrates lipid transport with hepatic uptake pathways and contributes to systemic energy homeostasis. Altered LIPC activity or expression has been linked to dyslipidemia phenotypes, including changes in HDL cholesterol levels and lipid particle distribution, making it a useful target for mechanistic studies of lipid metabolism. LIPC is also studied in the context of cardiometabolic risk pathways and gene-by-environment effects on plasma lipid profiles.
Hepatic Lipase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LIPC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LIPC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LIPC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LIPC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.