HCF1 CRISPR/Cas9 KO Plasmid (h): sc-403097
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HCF1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HCF1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HCF1 Antibody (H-8): sc-390950
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HCF1 CRISPR/Cas9 KO Plasmid (h) | sc-403097 | 20 µg | $397.00 |
Overview
HCFC1 encodes host cell factor 1 (HCF1), a chromatin-associated transcriptional co-regulator that bridges sequence-specific transcription factors with histone-modifying complexes to coordinate gene expression programs required for cell-cycle progression and cellular identity. HCF1 participates in epigenetic control of promoters and enhancers, supporting pathways linked to G1/S transition, DNA replication competence, and transcriptional homeostasis. Disruption of HCFC1 function has been implicated in neurodevelopmental phenotypes and X-linked intellectual disability, and altered HCF1-dependent transcriptional networks are also studied in the context of oncogenic proliferation. As a nexus for chromatin remodeling and transcription factor assembly, HCF1 is frequently interrogated in studies of promoter regulation, cell-cycle control, and genome-wide gene expression circuitry.
HCF1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HCFC1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HCFC1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HCFC1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HCF1 protein expression.
This CRISPR knockout system enables efficient generation of HCFC1-deficient cell models for investigation of HCF1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HCFC1 exon(s) critical for HCF1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HCFC1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HCF1 CRISPR/Cas9 KO Plasmid (h) and HCF1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HCFC1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HCF1 HDR Plasmid (h) and HCF1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HCFC1 homology arms to support homology-directed repair at defined HCFC1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.