H2-Dd Antibody (34-5-8S): sc-52542
- H2-Dd Antibody (34-5-8S) is a mouse monoclonal IgG2a H2-Dd antibody provided at 100 µg/ml
- raised against C57BL/6 x DBA/2 F1 hybrid splenocytes of mouse origin
- recommended for detection of a conformational epitope on H2-Dd MHC class I found on the N terminal domains of α1 and α2 chains when complexed with β2 microglobulin of mouse origin by IP and FCM; non cross-reactive with H2Dd α chains synthesized in vitro
- available conjugated to phycoerythrin for IF, IHC(P) and FCM
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for H2-Dd Antibody (34-5-8S). These reagents are now offered in bundles with H2-Dd Antibody (34-5-8S) (see ordering information below).
H2-Dd Antibody (34-5-8S) is a mouse monoclonal IgG2a antibody that detects H2-Dd protein of mouse origin by immunoprecipitation (IP) and flow cytometry (FCM). Anti-H2-Dd antibody (34-5-8S) is available as the non-conjugated format. H2-Dd protein is a crucial component of the major histocompatibility complex (MHC) class I molecules, which play a vital role in the immune response by presenting foreign antigens to cytotoxic T lymphocytes (CTLs). Located on the surface of cells, H2-Dd binds to peptides derived from intracellular proteins, allowing the immune system to monitor cellular health and detect infections or malignancies. The ability of H2-Dd to present a diverse array of peptides is essential for the adaptive immune response, as this enables recognition of a wide range of pathogens. Furthermore, H2-Dd interaction with specific receptors, such as murine Ly-49D and Ly-49A, highlights its importance in modulating immune responses and maintaining self-tolerance. Using H2-Dd monoclonal antibody (34-5-8S) in research can provide valuable insights into immune recognition mechanisms and MHC molecule roles in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
H2-Dd Antibody (34-5-8S) References:
- Activating Ly-49D and inhibitory Ly-49A natural killer cell receptors demonstrate distinct requirements for interaction with H2-D(d). | Nakamura, MC., et al. 2000. J Exp Med. 192: 447-54. PMID: 10934233
- Ligand interactions by activating and inhibitory Ly-49 receptors. | Nakamura, MC. and Seaman, WE. 2001. Immunol Rev. 181: 138-48. PMID: 11513135
- Peptide and beta 2-microglobulin regulation of cell surface MHC class I conformation and expression. | Otten, GR., et al. 1992. J Immunol. 148: 3723-32. PMID: 1602127
- Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens. | Evans, GA., et al. 1982. Nature. 300: 755-7. PMID: 6184620
- Cloning and functional characteristics of murine large granular lymphocyte-1: a member of the Ly-49 gene family (Ly-49G2). | Mason, LH., et al. 1995. J Exp Med. 182: 293-303. PMID: 7629495
- Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules. | Kane, KP. 1994. J Exp Med. 179: 1011-5. PMID: 8113669
- Recognition of class I major histocompatibility complex molecules by Ly-49: specificities and domain interactions. | Brennan, J., et al. 1996. J Exp Med. 183: 1553-9. PMID: 8666913
- The natural killer cell receptor Ly-49A recognizes a peptide-induced conformational determinant on its major histocompatibility complex class I ligand. | Orihuela, M., et al. 1996. Proc Natl Acad Sci U S A. 93: 11792-7. PMID: 8876216
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
H2-Dd Antibody (34-5-8S) | sc-52542 | 100 µg/ml | $322.00 | |||
H2-Dd Antibody (34-5-8S): m-IgG Fc BP-HRP Bundle | sc-538957 | 100 µg Ab; 10 µg BP | $361.00 | |||
H2-Dd Antibody (34-5-8S): m-IgG2a BP-HRP Bundle | sc-546652 | 100 µg Ab; 10 µg BP | $361.00 |