GRSF-1 CRISPR/Cas9 KO Plasmid (h): sc-405858
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GRSF-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GRSF-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRSF-1 CRISPR/Cas9 KO Plasmid (h) | sc-405858 | 20 µg | $397.00 |
Overview
GRSF1 encodes GRSF-1, an RNA-binding protein enriched in mitochondria that recognizes G-rich RNA elements and supports post-transcriptional control of mitochondrial gene expression. It participates in mitochondrial RNA processing, stability, and translation, thereby influencing oxidative phosphorylation, mitochondrial ribosome function, and cellular bioenergetics. Through these roles, GRSF-1 connects RNA metabolism to stress responses and maintenance of mitochondrial homeostasis. Altered GRSF1 activity has been investigated in contexts involving mitochondrial dysfunction, including neurodegeneration and cancer-associated metabolic remodeling, where mitochondrial gene-expression programs are frequently perturbed.
GRSF-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GRSF1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GRSF1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GRSF1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GRSF-1 protein expression.
This CRISPR knockout system enables efficient generation of GRSF1-deficient cell models for investigation of GRSF-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GRSF1 exon(s) critical for GRSF-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GRSF1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GRSF-1 CRISPR/Cas9 KO Plasmid (h) and GRSF-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GRSF1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GRSF-1 HDR Plasmid (h) and GRSF-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GRSF1 homology arms to support homology-directed repair at defined GRSF1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.