Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GRASP55 Double Nickase Plasmid (h): sc-401106-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GRASP55 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GRASP55 Double Nickase Plasmid (h) and GRASP55 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GORASP2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GRASP55 Antibody (E-5): sc-271840
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRASP55 Double Nickase Plasmid (h) | sc-401106-NIC | 20 µg | $410.00 | |||
GRASP55 Double Nickase Plasmid (h2) | sc-401106-NIC-2 | 20 µg | $410.00 |
Human GORASP2 encodes GRASP55, a Golgi reassembly stacking protein that helps maintain Golgi cisternal organization and supports vesicle tethering and membrane trafficking between the ER and Golgi. GRASP55 participates in Golgi ribbon formation and is implicated in unconventional protein secretion, contributing to proteostasis and stress-adaptive trafficking programs. Perturbation of GRASP55-dependent Golgi architecture can alter glycosylation, secretion, and cell signaling output, processes frequently remodeled in cancer, neurodegeneration, and inflammatory states. As a result, GORASP2 is a useful locus for dissecting how Golgi structure interfaces with secretory pathway homeostasis and disease-associated cellular phenotypes.
GRASP55 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GORASP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GORASP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GORASP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GORASP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.