Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GLUD1 Double Nickase Plasmid (h): sc-402187-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GLUD1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GLUD1 Double Nickase Plasmid (h) and GLUD1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GLUD1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GLUD1 Double Nickase Plasmid (h) | sc-402187-NIC | 20 µg | $410.00 | |||
GLUD1 Double Nickase Plasmid (h2) | sc-402187-NIC-2 | 20 µg | $410.00 |
GLUD1 encodes mitochondrial glutamate dehydrogenase 1, an enzyme that catalyzes the reversible oxidative deamination of glutamate to α-ketoglutarate and ammonia, linking amino acid catabolism to the tricarboxylic acid (TCA) cycle and cellular bioenergetics. By regulating the glutamate/α-ketoglutarate node, GLUD1 influences nitrogen handling, redox balance, and anaplerotic flux that supports oxidative metabolism. Its activity intersects with pathways controlling insulin secretion and neurotransmitter homeostasis through coupling of glutamate metabolism to ATP production and mitochondrial function. Dysregulation of GLUD1 has been associated with disorders of amino acid metabolism and neuroendocrine phenotypes, making it a relevant target for studying metabolic control in human cells.
GLUD1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GLUD1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GLUD1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GLUD1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GLUD1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.