GENIUS™ Nuclease DMF Filed (CAS 9025-65-4)

用法 :

Format: Lyophilized powder


Reconstitution: It is recommended to reconstitute the lyophilized GENIUS™ Nuclease DMF Filed in 100 μl (per 25KU) sterile 50% glycerol to a final concentration of 250U/μl. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing by hand. Avoid vigorous shaking or vortexing.


Removal of GENIUS™ Nuclease DMF Filed: GENIUS^TM Nuclease DMF Filed contain no "Tag" and used in downstream processing can be removed by various purification methods according to the purification strategy for the target protein.


Reaction buffer: 50 mM Tris-HCl, 1 mM MgCl2, pH 8.0. For dilute solutions, addition of a carrier protein such as 0.1 mg/ml HSA or BSA is recommended to avoid any enzyme loss from surface adsorption.


The activity of any unknown nuclease can be determined from a single measurement by means of the standard curve. The specific activity of GENIUS™ Nuclease DMF Filed is 1.3×10⁶ ~ 1.5×10⁶ unit/mg protein.

Lyophilized GENIUS™ Nuclease DMF Filed is shipped at Ambient temperature. Upon receipt, store lyophilized product at -20°C, stable for 1 year. Following reconstitution, store at -20°C to -70°C. Avoid repeated freeze-thaw cycles.

処方 :

≥250 units

外観 :

Powder

身体の状態 :

Solid

に由来する :

Recombinant protein expressed in Escherichia coli

保存方法 :

Store at -20° C