m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Fibrillarin Antibody (G-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with Fibrillarin Antibody (G-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Fibrillarin Antibody (G-8) is a high quality monoclonal Fibrillarin antibody (also designated FBL antibody) suitable for the detection of the Fibrillarin protein of mouse, rat and human origin. Fibrillarin Antibody (G-8) is available as both the non-conjugated anti-Fibrillarin antibody form, as well as multiple conjugated forms of anti-Fibrillarin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Fibrillarin is a widely occurring, basic, nonhistone protein that is localized exclusively in the fibrillar region of the nucleolus, including both the dense fibrillar and the fibrillar center regions. Fibrillarin is a protein that is also expressed in HeLa cells, 3T3 cells, and human peripheral blood lymphocytes. In metaphase and anaphase, fibrillarin is found on putative chromosomal nucleolar regions (NORs). During telophase, fibrillarin is an early marker for the site of the newly forming nucleolus. The structure of fibrillarin includes an RNA-binding domain and an RNP consensus sequence, which is consistent with the association of fibrillarin with the U3 small nucleolar RNA. Fibrillarin is involved in processing rRNA transcripts in the nucleolus.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
I was wondering whether you have any information regarding room temp incubation of this antibody (sc-374022) for IF on cells? I'm planning to use this for a drug screen and the system is not amenable to o/n incubation at 4C. Thanks.
Thank you for your question. Yes, we do recommend incubating with this antibody for 60 minutes at room temperature. Please refer to our immunofluorescence protocol for more detailed information.
回答者: Technical Support
What application is the blocking peptide sc-374022 P appropriate for?
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
Works ok for IF.akI got a sample of this antibody. Clear but weak IF with 1:50 dilution.
great features!Works well for immunofluorescence and Wester blotting in human HT1080 cancer cells and MEF cells.
an excellent antibody for both WB and IFThis antibody works very well for WB (40 ug U251 Lysate) and IF (formaldehyde-fixed HeLa cells) at 1:500 dilution.
Great for IF and co-localization experimentsI used a sample of this antibody for co-localization studies in mouse ES cells. The nucleolar staining was very specific and significant. generally pleased with outcome. Will recommend it!
Broad reactivity.This antibody is capable of detecting Fibrillarin multiple different species. Both staining and blotting assays provide clear and strong signal. This antibody has been used for publishing.
StrongStrong, clear bands present in Western blot analysis of Fibrillarin expression in HeLa and NIH/3T3 whole cell lysates. -SCBT QC
Superb immunoperoxidase nucleolar and nuclearSuperb immunoperoxidase nucleolar and nuclear staining in formalin fixed, paraffin-embedded human breast tissue. -SCBT QC