FAF1 CRISPR/Cas9 KO Plasmid (m): sc-420281
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAF1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FAF1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FAF1 Antibody (E-4): sc-393965
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAF1 CRISPR/Cas9 KO Plasmid (m) | sc-420281 | 20 µg | $397.00 |
Overview
Fas-associated factor 1 (FAF1), encoded by the mouse Faf1 gene, is a multifunctional adaptor implicated in regulation of apoptosis and protein quality control. FAF1 participates in death receptor–associated signaling complexes and modulates caspase-dependent pathways, while also interacting with ubiquitin–proteasome system components to influence ubiquitination and turnover of signaling proteins. Through these activities, FAF1 links stress responses to cell fate decisions and contributes to maintenance of cellular homeostasis. Dysregulated FAF1 expression or function has been associated with altered apoptotic sensitivity and has been investigated in contexts including neurodegeneration, immune regulation, and cancer-related signaling.
FAF1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Faf1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Faf1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Faf1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FAF1 protein expression.
This CRISPR knockout system enables efficient generation of Faf1-deficient cell models for investigation of FAF1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Faf1 exon(s) critical for FAF1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Faf1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FAF1 CRISPR/Cas9 KO Plasmid (m) and FAF1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Faf1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FAF1 HDR Plasmid (m) and FAF1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Faf1 homology arms to support homology-directed repair at defined Faf1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.