ER71 CRISPR Activation Plasmid (m): sc-420239-ACT
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ER71 CRISPR Activation Plasmid (m) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- ER71 CRISPR Activation Plasmid (m) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by ER71 CRISPR Activation Plasmid (m) and ER71 CRISPR Activation Plasmid (m2) target distinct regulatory regions upstream of the Etv2 transcriptional start site. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ER71 CRISPR Activation Plasmid (m) | sc-420239-ACT | 20 µg | $397.00 |
Mouse Etv2 encodes the ETS family transcription factor ER71, a master regulator of hematoendothelial specification during early embryogenesis. ER71 integrates developmental signaling inputs to control transcriptional programs that drive vascular morphogenesis, endothelial differentiation, and hematopoietic lineage emergence, including regulation of key endothelial gene networks. Etv2 activity intersects with pathways governing angiogenesis and vasculogenesis, and its dysregulation is used to model defects in cardiovascular development and aberrant endothelial identity in experimental systems. Because Etv2 is transiently expressed and tightly dosage-controlled, it is widely studied in reprogramming paradigms and lineage-tracing workflows that interrogate endothelial and blood progenitor fate decisions.
ER71 CRISPR Activation Plasmid (m) provides a targeted, non-destructive approach to upregulating endogenous Etv2 expression without altering the underlying DNA sequence.
ER71 CRISPR Activation Plasmid (m) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the Etv2 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the Etv2 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous ER71 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native Etv2 locus and enabling the study of ER71-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of ER71 pathway restoration in tumor cells with silenced or reduced Etv2 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.