Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ECM1 Double Nickase Plasmid (m): sc-420098-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ECM1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ECM1 Double Nickase Plasmid (m) and ECM1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ecm1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ECM1 Antibody (F-1): sc-365335
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ECM1 Double Nickase Plasmid (m) | sc-420098-NIC | 20 µg | $410.00 | |||
ECM1 Double Nickase Plasmid (m2) | sc-420098-NIC-2 | 20 µg | $410.00 |
Mouse Ecm1 encodes extracellular matrix protein 1 (ECM1), a secreted glycoprotein that helps organize extracellular matrix architecture and modulate cell–matrix signaling. ECM1 interacts with matrix components and proteases to influence basement membrane integrity, extracellular remodeling, and tissue homeostasis, impacting processes such as adhesion, migration, and differentiation. Altered ECM1 expression or function has been associated with dysregulated matrix deposition and inflammatory microenvironments, making it relevant to studies of skin and connective tissue biology as well as stromal regulation in disease models.
ECM1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ecm1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ecm1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ecm1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ecm1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.