Date published: 2026-5-7

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E2F-4 Antibody (WUF2): sc-69685

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Datasheets
  • E2F-4 Antibody (WUF2) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
  • raised against recombinant E2F-4 of human origin
  • recommended for detection of E2F-4 of mouse, rat and human origin by WB and IP
  • See E2F-4 (D-7): sc-398543 for E2F-4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for E2F-4 Antibody (WUF2) for WB applications. These reagents are now offered in bundles with E2F-4 Antibody (WUF2) (see ordering information below).
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    E2F-4 Antibody (WUF2) is a mouse monoclonal IgG antibody that detects E2F-4 in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). E2F-4 is a crucial member of the E2F family of transcription factors, which are integral to the regulation of the cell cycle, particularly during the transition from the G1 phase to the S phase. E2F-4 functions primarily as a transcriptional repressor, playing a vital role in controlling the expression of genes necessary for cell proliferation and differentiation. E2F-4 forms heterodimers with the DP-1 protein, which is essential for the formation of an active transcriptional complex that regulates target gene expression. Dysregulation of E2F-4 can lead to uncontrolled cell growth and has been implicated in various cancers, making E2F-4 a significant focus of research in understanding tumorigenesis. Anti-E2F-4 antibody (WUF2) is an invaluable tool for studying the mechanisms of cell cycle regulation and potential therapeutic targets in cancer treatment.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    E2F-4 Antibody (WUF2)

    sc-69685
    200 µg/ml
    $322.00

    E2F-4 Antibody (WUF2): m-IgG Fc BP-HRP Bundle

    sc-539220
    200 µg Ab; 10 µg BP
    $361.00

    E2F-4 Antibody (WUF2): m-IgG1 BP-HRP Bundle

    sc-541334
    200 µg Ab; 20 µg BP
    $361.00

    What blocking solution should I use with E2F-4 (WUF2): sc-69685 monoclonal antibody for Western blot?

    Asked by: Cweed
    Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 4 out of 5 by from Worked well in WB in Y79 nuclear extractWorked well in WB in Y79 nuclear extract. -SCBT QC
    Date published: 2015-03-22
    Rated 4 out of 5 by from Single strong band in WB in mouse transfectedSingle strong band in WB in mouse transfected 293T whole cell lysates. -SCBT QC
    Date published: 2015-02-16
    Rated 4 out of 5 by from Good WB specificity in HLGood WB specificity in HL-60, Raji and Jurkat whole cell lysates. -SCBT QC
    Date published: 2014-09-14
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    E2F-4 Antibody (WUF2) is rated 4.0 out of 5 by 3.
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