Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Dvl-1/Dishevelled 1/DVL1 Double Nickase Plasmid (h): sc-400785-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Dvl-1/Dishevelled 1/DVL1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Dvl-1/Dishevelled 1/DVL1 Double Nickase Plasmid (h) and Dvl-1/Dishevelled 1/DVL1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DVL1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Dvl-1/Dishevelled 1/DVL1 Antibody (3F12): sc-8025
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dvl-1/Dishevelled 1/DVL1 Double Nickase Plasmid (h) | sc-400785-NIC | 20 µg | $410.00 | |||
Dvl-1/Dishevelled 1/DVL1 Double Nickase Plasmid (h2) | sc-400785-NIC-2 | 20 µg | $410.00 |
DVL1 encodes Dishevelled 1 (Dvl-1), a central cytoplasmic scaffold that couples Frizzled receptors to downstream Wnt signaling. Through regulated interactions and phosphorylation, DVL1 coordinates canonical Wnt/β-catenin transcriptional programs as well as noncanonical planar cell polarity and Wnt/Ca²⁺ pathways that shape cytoskeletal dynamics, polarity, and cell migration. DVL1 activity influences developmental patterning, stem-like cell behavior, and tissue homeostasis, and aberrant Wnt–Dishevelled signaling is frequently associated with dysregulated proliferation and invasion in cancer biology. These properties make DVL1 a useful target for dissecting pathway crosstalk, signalosome assembly, and context-specific Wnt outputs in human cells.
Dvl-1/Dishevelled 1/DVL1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DVL1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DVL1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DVL1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DVL1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.