DRP1 Double Nickase Plasmid (h): sc-400459-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DRP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DRP1 Double Nickase Plasmid (h) and DRP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DNM1L. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DRP1 Antibody (C-5): sc-271583
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DRP1 Double Nickase Plasmid (h) | sc-400459-NIC | 20 µg | $410.00 | |||
DRP1 Double Nickase Plasmid (h2) | sc-400459-NIC-2 | 20 µg | $410.00 |
DNM1L encodes dynamin-related protein 1 (DRP1), a large GTPase that orchestrates mitochondrial fission by assembling on the outer mitochondrial membrane and constricting membranes in coordination with adaptor proteins such as FIS1, MFF, and MID49/51. Through regulation of mitochondrial network architecture, DRP1 influences oxidative phosphorylation, reactive oxygen species handling, mitochondrial DNA distribution, and organelle quality control via mitophagy. DRP1-dependent dynamics are tightly coupled to apoptosis signaling and metabolic adaptation, linking DNM1L activity to pathways governing cellular stress responses and bioenergetic homeostasis. Dysregulated DRP1 function and mitochondrial fragmentation are associated with neurodevelopmental and neurodegenerative phenotypes, cardiometabolic dysfunction, and cancer cell survival programs, making DNM1L a common target for mechanistic studies of mitochondrial biology.
DRP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DNM1L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DNM1L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DNM1L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DNM1L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.