DPF2 Double Nickase Plasmid (h): sc-404801-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DPF2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DPF2 Double Nickase Plasmid (h) and DPF2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DPF2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DPF2 Antibody (C-9): sc-514297
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DPF2 Double Nickase Plasmid (h) | sc-404801-NIC | 20 µg | $410.00 | |||
DPF2 Double Nickase Plasmid (h2) | sc-404801-NIC-2 | 20 µg | $410.00 |
DPF2 (double PHD fingers 2; also known as BAF45d) is a chromatin-associated adaptor that reads histone marks through its PHD zinc fingers and helps target SWI/SNF (BAF) chromatin remodeling complexes to specific genomic loci. By coupling epigenetic signals to nucleosome repositioning, DPF2 contributes to transcriptional regulation programs that shape cell cycle control, differentiation, and stimulus-responsive gene expression. DPF2-dependent chromatin remodeling intersects with pathways governing developmental gene networks and DNA damage–associated transcriptional responses. Dysregulation of SWI/SNF components and chromatin reader functions, including DPF2-associated activities, is frequently studied in the context of altered transcriptional states observed in cancer and other disorders with epigenetic perturbation.
DPF2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DPF2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DPF2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DPF2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DPF2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.