DDX1 Double Nickase Plasmid (h): sc-405137-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDX1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DDX1 Double Nickase Plasmid (h) and DDX1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DDX1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DDX1 Antibody (A-7): sc-271438
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX1 Double Nickase Plasmid (h) | sc-405137-NIC | 20 µg | $410.00 | |||
DDX1 Double Nickase Plasmid (h2) | sc-405137-NIC-2 | 20 µg | $410.00 |
DDX1 (DEAD-box helicase 1) is a human ATP-dependent RNA helicase that remodels RNA and ribonucleoprotein complexes to support RNA processing, transport, and translation. It participates in transcription-associated RNA metabolism and contributes to maintenance of genome integrity through interactions with DNA damage response and repair machinery. DDX1 has also been implicated in innate immune signaling through regulation of RNA sensing pathways and stress-responsive ribonucleoprotein granules. Dysregulated DDX1 activity or expression has been associated with altered proliferation and RNA homeostasis in cancer-relevant contexts, making it a useful node for mechanistic studies of RNA helicase function.
DDX1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DDX1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DDX1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DDX1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DDX1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.