Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DDB2 Double Nickase Plasmid (h): sc-401686-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDB2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DDB2 Double Nickase Plasmid (h) and DDB2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DDB2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DDB2 Antibody (2246C4a): sc-81246
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDB2 Double Nickase Plasmid (h) | sc-401686-NIC | 20 µg | $410.00 | |||
DDB2 Double Nickase Plasmid (h2) | sc-401686-NIC-2 | 20 µg | $410.00 |
DDB2 encodes a DNA damage recognition factor that partners with DDB1 to form the UV-DDB complex, a key sensor of UV-induced photolesions that initiates global genome nucleotide excision repair. Through its coupling to the CUL4A–RBX1 E3 ubiquitin ligase machinery, DDB2 promotes lesion verification and chromatin remodeling by ubiquitinating repair-associated substrates, thereby facilitating recruitment of downstream NER components. DDB2 activity intersects with cell-cycle checkpoint control and genome stability pathways, linking defective damage recognition to increased mutational burden. Altered DDB2 expression or function has been associated with UV sensitivity phenotypes and has been studied in the context of carcinogenesis and DNA repair–related disorders.
DDB2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DDB2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DDB2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DDB2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DDB2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.