DACH1 Double Nickase Plasmid (h): sc-404712-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DACH1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DACH1 Double Nickase Plasmid (h) and DACH1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DACH1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DACH1 Antibody (A-6): sc-398706
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DACH1 Double Nickase Plasmid (h) | sc-404712-NIC | 20 µg | $410.00 | |||
DACH1 Double Nickase Plasmid (h2) | sc-404712-NIC-2 | 20 µg | $410.00 |
DACH1 (dachshund family transcription factor 1) is a nuclear DNA-binding transcriptional regulator that modulates cell fate decisions during development and maintains tissue-specific differentiation programs in adult cells. It influences gene expression networks linked to cell-cycle control, epithelial–mesenchymal balance, and lineage specification through interactions with cofactors and chromatin-regulatory complexes. DACH1 activity has been connected to pathways governing proliferation and migration, including crosstalk with hormone receptor signaling and growth factor–responsive transcriptional programs. Altered DACH1 expression or regulatory context has been reported across multiple tumor types and is also relevant to studies of organogenesis and congenital patterning phenotypes.
DACH1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DACH1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DACH1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DACH1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DACH1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.