cyclin D1 Antibody (A-12): sc-8396
- cyclin D1 Antibody (A-12) is a mouse monoclonal IgG2b κ cyclin D1 antibody, cited in 732 publications, provided at 200 µg/ml
- raised against amino acids 1-295 representing full length cyclin D1 of human origin
- cyclin D1 Antibody (A-12) is recommended for detection of cyclin D1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; may cross-react with cyclin D2; non cross-reactive with cyclin D3
- Anti-cyclin D1 Antibody (A-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to TRITC for IF, IHC(P) and FCM
- m-IgG2b BP-HRP is the preferred secondary detection reagent for cyclin D1 Antibody (A-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with cyclin D1 Antibody (A-12) (see ordering information below).
QUICK LINKS
Cyclin D1 Antibody (A-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects cyclin D1 of mouse, rat, and human origin through various applications including western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, flow cytometry (FCM), and enzyme-linked immunosorbent assay. Cyclin D1 (A-12) antibody is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. Cyclin D1 plays a pivotal role in cell cycle regulation, particularly during the G1 phase, where cyclin D1 is essential for the transition from G1 to S phase. This transition is crucial for cellular proliferation, as cells prepare for DNA synthesis. Dysregulation of cyclin D1 is associated with various cancers, leading to uncontrolled cell division. Cyclin D1 binds and activates cyclin-dependent kinases (CDKs), facilitating phosphorylation of the retinoblastoma protein, which releases E2F transcription factors that drive expression of genes necessary for DNA replication. Understanding cyclin D1 function is vital for elucidating mechanisms of tumorigenesis and developing targeted cancer therapies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cyclin D1 Antibody (A-12) References:
- ras activity and cyclin D1 expression: an essential mechanism of mouse skin tumor development. | Rodriguez-Puebla, ML., et al. 1999. Mol Carcinog. 24: 1-6. PMID: 10029404
- Cyclin D1 in breast premalignancy and early breast cancer: implications for prevention and treatment. | Zhou, Q., et al. 2001. Cancer Lett. 162: 3-17. PMID: 11121857
- Cyclin D1: mechanism and consequence of androgen receptor co-repressor activity. | Petre, CE., et al. 2002. J Biol Chem. 277: 2207-15. PMID: 11714699
- Cyclin A in cell cycle control and cancer. | Yam, CH., et al. 2002. Cell Mol Life Sci. 59: 1317-26. PMID: 12363035
- Cyclin D1 repression of peroxisome proliferator-activated receptor gamma expression and transactivation. | Wang, C., et al. 2003. Mol Cell Biol. 23: 6159-73. PMID: 12917338
- Cyclin D1 gene (CCND1) mutations in endometrial cancer. | Moreno-Bueno, G., et al. 2003. Oncogene. 22: 6115-8. PMID: 12955092
- Cyclin D1 induction of cellular migration requires p27(KIP1). | Li, Z., et al. 2006. Cancer Res. 66: 9986-94. PMID: 17047061
- Cyclin D1 regulates hepatic estrogen and androgen metabolism. | Mullany, LK., et al. 2010. Am J Physiol Gastrointest Liver Physiol. 298: G884-95. PMID: 20338923
- Cyclin D1 inactivation extends proliferation and alters histogenesis in the postnatal mouse retina. | Das, G., et al. 2012. Dev Dyn. 241: 941-52. PMID: 22434780
- Cyclin D1 overexpression in proliferation centres of small lymphocytic lymphoma/chronic lymphocytic leukaemia. | Teixeira Mendes, LS., et al. 2017. J Clin Pathol. 70: 899-902. PMID: 28408436
- Identifying Cyclin A/Cdk1 Substrates in Mitosis in Human Cells. | Dumitru, AMG. and Compton, DA. 2022. Methods Mol Biol. 2415: 175-182. PMID: 34972954
- Mechanism of cyclin D1 (CCND1, PRAD1) overexpression in human cancer cells: analysis of allele-specific expression. | Hosokawa, Y. and Arnold, A. 1998. Genes Chromosomes Cancer. 22: 66-71. PMID: 9591636
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin D1 Antibody (A-12) | sc-8396 | 200 µg/ml | $322.00 | |||
cyclin D1 Antibody (A-12): m-IgG2b BP-HRP Bundle | sc-548643 | 200 µg Ab; 10 µg BP | $361.00 | |||
cyclin D1 Antibody (A-12) AC | sc-8396 AC | 500 µg/ml, 25% agarose | $424.00 | |||
cyclin D1 Antibody (A-12) HRP | sc-8396 HRP | 200 µg/ml | $322.00 | |||
cyclin D1 Antibody (A-12) FITC | sc-8396 FITC | 200 µg/ml | $336.00 | |||
cyclin D1 Antibody (A-12) PE | sc-8396 PE | 200 µg/ml | $349.00 | |||
cyclin D1 Antibody (A-12) Alexa Fluor® 488 | sc-8396 AF488 | 200 µg/ml | $364.00 | |||
cyclin D1 Antibody (A-12) Alexa Fluor® 546 | sc-8396 AF546 | 200 µg/ml | $364.00 | |||
cyclin D1 Antibody (A-12) Alexa Fluor® 594 | sc-8396 AF594 | 200 µg/ml | $364.00 | |||
cyclin D1 Antibody (A-12) Alexa Fluor® 647 | sc-8396 AF647 | 200 µg/ml | $364.00 | |||
cyclin D1 Antibody (A-12) Alexa Fluor® 680 | sc-8396 AF680 | 200 µg/ml | $364.00 | |||
cyclin D1 Antibody (A-12) Alexa Fluor® 790 | sc-8396 AF790 | 200 µg/ml | $364.00 | |||
cyclin D1 Antibody (A-12) TRITC | sc-8396 TRITC | 200 µg/ml | $326.00 |