Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CUL-2 Double Nickase Plasmid (h): sc-402370-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CUL-2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CUL-2 Double Nickase Plasmid (h) and CUL-2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CUL2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CUL-2 Antibody (C-4): sc-166506
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CUL-2 Double Nickase Plasmid (h) | sc-402370-NIC | 20 µg | $410.00 | |||
CUL-2 Double Nickase Plasmid (h2) | sc-402370-NIC-2 | 20 µg | $410.00 |
Human CUL2 encodes cullin-2 (CUL-2), a core scaffold of CUL2-RING E3 ubiquitin ligase complexes that couple substrate recognition modules such as VHL or SOCS-box proteins to RBX1-mediated ubiquitin transfer. These complexes regulate proteostasis by targeting specific proteins for ubiquitination and proteasomal degradation, shaping pathways that include oxygen-sensing and hypoxia-inducible factor (HIF) signaling, cell-cycle progression, and DNA damage responses. Through its role in VHL-dependent turnover of HIF-α and other substrates, CUL-2 helps coordinate cellular adaptation to oxygen availability and metabolic state. Dysregulation of CUL2-dependent ubiquitination networks has been linked to oncogenic signaling, altered hypoxia responses, and genome stability defects relevant to cancer biology.
CUL-2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CUL2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CUL2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CUL2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CUL2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.