Date published: 2026-5-3

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Control 293T Lysate: sc-117752

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Datasheets
  • 100 µg protein in 200 µl SDS-PAGE buffer
  • Western Blot negative control lysate derived from non-transfected 293T cells
  • Suitable for use with our 293T Transfected Lysates
  • Recommended use: 10-20 µl per lane
  • sample vial should be placed at 95° C for up to 5 minutes, once prior to use
  • sample vial should be boiled once prior to use
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293T Control Lysate is derived from the 293T cell line, a human embryonic kidney cell line genetically modified to express the SV40 large T antigen. This lysate is widely used as a reference sample in various research applications to ensure the accuracy and reproducibility of experimental results. 293T cells are known for their high transfection efficiency, making them an ideal system for studying gene expression, protein-protein interactions, and cellular signaling pathways. The control lysate contains a comprehensive mix of cytoplasmic and nuclear proteins, allowing researchers to analyze the expression and modification of proteins under standard conditions. In research, 293T Control Lysate is utilized as a baseline to compare against experimental samples. It is essential in techniques such as Western blotting, where it serves as a positive control to verify the presence and size of target proteins. Additionally, it is used in immunoprecipitation assays to confirm antibody specificity and in mass spectrometry to identify and quantify proteins. By providing a consistent and well-characterized sample, 293T Control Lysate helps researchers standardize their experiments, ensuring that observed effects are due to experimental variables rather than inconsistencies in sample preparation. The origin and quality of the 293T cell line are rigorously maintained to provide reliable and reproducible results in basic scientific research.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Control 293T Lysate

sc-117752
100 µg/200 µl
$120.00