Thank you for your question. What dilution we use depends on the application it is being used for, as well as sample type and secondary reagents, but here is a general guideline of starting dilutions (further titration/optimization will be required):
Western blot: 1:100
Immunofluorescence/IHC: 1:50
Most other applications, we recommend doing a titration.
回答者: Tech Service
投稿日: 2022-09-27
what is the best blocking agent for this antibody BSA or fat free milk and in which buffer
質問者: 匿名
Thank you for your question.
Which blocking reagent depends on which application you are using the antibody for. For Western blot we recommend 5% milk with TBS-T. For IHC and IF applications we recommend using blocking serum derived from the same species in which the secondary antibody is raised.
David Jenによって、
5つ星のうち
5と評価されています。
sc-8784 quality for research.It works very well in the Western blot analysis. We recommend it to use.
投稿日: 2017-07-18
kdd246によって、
5つ星のうち
5と評価されています。
Perfect functionwe use this antibody nearly one year, it works very well, and it is trustworthy.
投稿日: 2016-11-28
Hao_Xuによって、
5つ星のうち
4と評価されています。
Quite strong in mouse arteriesQuite strong in mouse arteries, Band of interest seen clearly plus some other bands.
投稿日: 2015-09-23
Jianによって、
5つ星のうち
5と評価されています。
Worked well for WB in mouseWorked well for WB in mouse.
投稿日: 2015-08-21
SMによって、
5つ星のうち
5と評価されています。
Worked well with murine tissueWorked well with murine tissue.
投稿日: 2015-06-19
Odinugaによって、
5つ星のうち
5と評価されています。
This antibody has been working well for me so farThis antibody has been working well for me so far. This is our second time ordering it and we have used it in WB and IF and obtained great results.
投稿日: 2015-06-04
Rayeによって、
5つ星のうち
5と評価されています。
No commentNo comment
投稿日: 2015-03-23
BOによって、
5つ星のうち
5と評価されています。
Worked well for IF with methanolWorked well for IF with methanol-fixed HS68 cells. -SCBT QC