Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CNPase Double Nickase Plasmid (h): sc-402463-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CNPase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CNPase Double Nickase Plasmid (h) and CNPase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CNP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CNPase Antibody (H-2): sc-166558
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CNPase Double Nickase Plasmid (h) | sc-402463-NIC | 20 µg | $410.00 | |||
CNPase Double Nickase Plasmid (h2) | sc-402463-NIC-2 | 20 µg | $410.00 |
Human CNP encodes 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase), a myelin-associated enzyme enriched in oligodendrocytes and Schwann cells that hydrolyzes 2′,3′-cyclic nucleotides to 2′-nucleotides. CNPase supports myelin sheath formation and maintenance by influencing oligodendroglial process outgrowth, cytoskeletal dynamics, and RNA-associated ribonucleoprotein trafficking at the plasma membrane. Through these functions, CNPase contributes to axon–glia interactions and the stability of compact and non-compact myelin domains in the nervous system. Altered CNP expression or CNPase activity has been linked to demyelination, white matter dysfunction, and neuroinflammatory contexts, making the pathway relevant for mechanistic studies of myelin integrity and neural circuit stability.
CNPase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CNP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CNP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CNP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CNP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.