CLCA1 Double Nickase Plasmid (h): sc-402998-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CLCA1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CLCA1 Double Nickase Plasmid (h) and CLCA1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CLCA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CLCA1 Antibody (E-4): sc-271156
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLCA1 Double Nickase Plasmid (h) | sc-402998-NIC | 20 µg | $410.00 | |||
CLCA1 Double Nickase Plasmid (h2) | sc-402998-NIC-2 | 20 µg | $410.00 |
CLCA1 (chloride channel accessory 1) encodes a secreted and membrane-associated glycoprotein that modulates epithelial chloride conductance and mucus physiology, particularly in the gastrointestinal and airway mucosa. It is commonly linked to goblet cell differentiation and regulation of mucin production, integrating with inflammatory and epithelial remodeling programs that shape barrier function. Altered CLCA1 expression has been observed in mucosal inflammatory states and in epithelial malignancies, where it is often used as a marker of differentiation status and changes in secretory lineage composition. These properties make CLCA1 a useful target for studying epithelial ion transport regulation, mucus homeostasis, and inflammation-associated transcriptional networks.
CLCA1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CLCA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CLCA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CLCA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CLCA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.