Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ceruloplasmin Double Nickase Plasmid (m): sc-419776-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ceruloplasmin Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ceruloplasmin Double Nickase Plasmid (m) and ceruloplasmin Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Cp. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ceruloplasmin Antibody (H-3): sc-365205
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ceruloplasmin Double Nickase Plasmid (m) | sc-419776-NIC | 20 µg | $410.00 | |||
ceruloplasmin Double Nickase Plasmid (m2) | sc-419776-NIC-2 | 20 µg | $410.00 |
Mouse Cp encodes ceruloplasmin, a secreted multicopper ferroxidase that oxidizes Fe2+ to Fe3+ to support transferrin loading and systemic iron trafficking. Through cooperation with ferroportin-dependent iron export, ceruloplasmin helps maintain cellular iron homeostasis, limits redox-active iron accumulation, and influences oxidative stress responses. Ceruloplasmin activity intersects with copper metabolism and acute-phase inflammatory programs, linking hepatocyte secretion and macrophage iron handling to broader metabolic regulation. Dysregulation of Cp is used to model perturbations in iron/copper balance and oxidative injury relevant to neurobiology, liver physiology, and inflammatory pathology in mouse systems.
ceruloplasmin Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Cp locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Cp. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Cp function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Cp-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.