Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CEACAM1 CRISPR/Cas9 KO Plasmid (h): sc-417666
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CEACAM1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CEACAM1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CEACAM1 Antibody (E-1): sc-166453
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CEACAM1 CRISPR/Cas9 KO Plasmid (h) | sc-417666 | 20 µg | $397.00 |
Overview
CEACAM1 (carcinoembryonic antigen-related cell adhesion molecule 1) is an immunoglobulin superfamily cell-surface glycoprotein that mediates homophilic and heterophilic adhesion at epithelial and endothelial interfaces. Through its cytoplasmic ITIM motifs, CEACAM1 modulates phosphatase-dependent signaling to influence MAPK and PI3K/AKT pathway output, cytoskeletal organization, and junctional integrity. It also functions as an immune regulatory receptor on leukocytes, shaping activation thresholds and cytokine responses in inflammatory microenvironments. Dysregulated CEACAM1 expression and isoform balance have been associated with altered barrier function, immune evasion, angiogenic remodeling, and progression phenotypes across multiple solid tumors and inflammatory diseases.
CEACAM1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CEACAM1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CEACAM1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CEACAM1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CEACAM1 protein expression.
This CRISPR knockout system enables efficient generation of CEACAM1-deficient cell models for investigation of CEACAM1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CEACAM1 exon(s) critical for CEACAM1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CEACAM1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CEACAM1 CRISPR/Cas9 KO Plasmid (h) and CEACAM1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CEACAM1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CEACAM1 HDR Plasmid (h) and CEACAM1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CEACAM1 homology arms to support homology-directed repair at defined CEACAM1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.